题目：Improving cancer detection via nanoscale imaging of nuclear architecture
时间：2016.11.28, 下午4:00-5:00
地点：武汉光电国家实验室G301
邀请人：袁菁
主讲人：匹兹堡大学LIU Yang教授
Abstract:
Pathology remains the gold standard for cancer diagnosis and in some cases prognosis, in which trained pathologists examine abnormality in tissue architecture and cell morphology characteristic of cancer cells with a bright-field microscope. The limited microscale resolution of conventional microscope can result in intra-observer variation, missed early-stage cancers, and indeterminate cases that often result in unnecessary invasive procedures in the absence of cancer. We hypothesize that the assessment of nanoscale structural characteristics in the cell nucleus can potentially improve the sensitivity of cancer detection prior to the detection of clinically significant lesions. Towards this goal, we have developed several optical microscopy systems to interrogate nanoscale nuclear architecture. I will present the development of reflection-mode quantitative phase microscopy for depth-resolved nanoscale nuclear architecture mapping (nanoNAM) of clinically prepared formalin-fixed, paraffin-embedded tissue sections to detect the structural changes in nuclear architecture, not be easily identifiable by conventional pathology (Cancer Res, 2015; 75, 4718-4722). We will present the use of nanoNAM to detect cancer at the early stage of cancer development and predict "future" cancer progression in patients with inflammatory bowel disease. Further, the use of super-resolution fluorescence microscopy further elucidates the molecular-level changes of chromatin organization in cancer progression.
