【报告通知】Bessel Structured Light-sheet Imaging in Deep Tissue-Britton Chance 生物医学光子学研究中心 | Britton Chance Center for Biomedical Photonics




通知通告

新闻类别:通知通告
2017-05-24

【报告通知】Bessel Structured Light-sheet Imaging in Deep Tissue

报告题目:Bessel Structured Light-sheet Imaging in Deep Tissue
报告时间:2017年5月31号(周三)2:30 - 4:00
报告地点:武汉光电国家实验室A302
报 告 人:Dr. Leilei Peng  Associate Professor of Optical Sciences  Assistant Professor of Molecular and Cellular Biology
The University of Arizona, US
邀 请 人:袁  菁 教授

简介:分别于1997年、2000年在中国科技大学取得学士学位和硕士学位,2005年于美国Purdue University取得博士学位,2005-2009年在Harvard Medical School, Massachusetts General Hospital进行博士后训练,2009年起在University of Arizona任教。研究兴趣包括寿命光谱及显微成像,单分子成像和超分辨率显微成像。

Abstract:
In vivo fluorescent cellular imaging of deep tissue is highly challenging, because the excitation needs to penetrate through strong scattering tissue and the emission signal is degraded significantly by photon diffusion induced by tissue-scattering. To battle the challenge, our group combined Bessel light-sheet microscopy with nonlinear structured illumination microscopy (SIM) to produce cellular-resolution diffusion-free image of deep structures. With two-photon Bessel SIM light sheet excitation, tissue up to 600 microns wide can be imaged with 500 microns penetration depth, and clear images can be obtained at cellular resolution in depth beyond 200 microns, allowing noninvasive studying of deep organ in live embryos. With one-photon excitation, the spectral Bessel SIM light sheet technique can perform hyper-spectral imaging with true parallel detection on multiple excitation channels, which opens the door to fast multiplexed light-sheet imaging in live tissue.