Li L, Li M, Zhang Z, Huang ZL*, Assessing low-light cameras with photon transfer curve method, Journal of Innovative Optical Health Sciences, 2016 May;9(03):1630008. [PDF]          

We first present a brief explanation on the key parameters for characterizing low-light cameras, then explain the experimental procedures on how to measure PTC. We also show the application of the PTC method in experimentally quantifying the performance of two representative low-light cameras. Finally, we extend the PTC method to provide offset map, read noise map, and gain map of individual pixels inside a camera.

Gong WL, Xiong ZJ, Xin B, Yin H, Duan JS, Yan J, Chen T, Hua QX, Hu B, Huang ZL, Zhu MQ*, Twofold photoswitching of NIR fluorescence and EPR based on the PMI–N–HABI for optical nanoimaging of electrospun polymer nanowires. Journal of Materials Chemistry C. 2016;4(13):2498-505.             [PDF]          

we report the design and synthesis of a fluorescent molecular switch based on perylenemonoimide–hexaarylbiimidazole dyad (PMI–N–HABI), which exhibits not only photoswitchable nearinfrared (NIR) fluorescence but also photoswitchable electron paramagnetic resonance (EPR) resulting from photo-induced twin radicals.

J. Yan, L. X. Zhao, C. Li, Z.  Hu, G. F. Zhang, Z. Q. Chen, T. Chen, Z. L. Huang, J. T. Zhu, M. Q. Zhu ^*, Optical nanoimaging for block copolymer self-assembly, Journal of the American Chemical Society, 2015, 137(7):2436-2439. [PDF]        

We use STORM to get the optical nanoimaging of microphase structures of block copolymer self-assembly at 50 nm resolution.Block copolymer micelles are visualized in optical nanoimaging by staining the polystyrene blocks with spiropyrans (SPs),which exhibit reversible fluorescence on-off switching at alternating irradiation of UV and visible light.

D. Pan, Z. Hu, F. W. Qiu, Z. L. Huang, Y. L. Ma, Y. N. Wang, L. Q. Qin, Z. H. Zhang, S. Q. Zeng, Y. H. Zhang ^*, A general strategy for developing cell-permeable photo-modulatable organic fluorescent probes for live-cell super-resolution imaging, Nature Communications, 2014, 5: 5573. [PDF]        

It is a general strategy for constructing fluorescent probes for live-cell super-resolution imaging by exploiting the remarkable cytosolic delivery ability of a cell-penetrating peptide (rR)3R2. Our results indicate that these organic probes are not only cell permeable but can also specifically and directly label endogenous targeted proteins.

C. Li, H. Yan, L. X. Zhao, G. F. Zhang, Z. Hu, Z. L. Huang, M. Q. Zhu ^*, A trident dithienylethene-perylenemonoimide dyad with super fluorescence switching speed and ratio, Nature Communications, 2014, 5: 5709. [PDF]    

We report a trident perylenemonoimide modified by diethienylethenes shows a large on\off ratio and high switching speed, which enable erasable fluorescence patterning, all-optical transistor and optical nanoimaging.

F. Long, S. Q. Zeng, Z. L. Huang ^*, Effects of fixed pattern noise on single molecule localization microscopy, Physical Chemistry Chemical Physics, 2014, 16, 21586-21594. [PDF]    

A quantitative investigation on the effects of Fix pattern noise on localization precision and bias of single molecule localization microscopy.

Y. N. Wang, J. Schnitzbauer, Z. Hu, X. M. Li, Y. F. Cheng, Z. L. Huang, B. Huang ^* Localization events-based sample drift correction for localization microscopy with redundant cross-correlation algorithm, Optics Express, 2014, 22, 15982-15991. [PDF]        

we present a localization events-based drift correction method using a redundant cross-correlation algorithm originally developed to correct beaminduced motion in cryo-electron microscopy.

M. Q. Zhu ^*, G. F. Zhang ^§, Z. Hu ^§, M. P. Aldred, C. Li, W. L. Gong, T. Chen, Z. L. Huang ^*, S. Y. Liu ^* Reversible Fluorescence Switching of Spiropyran-Conjugated Biodegradable Nanoparticles for Super-Resolution Fluorescence Imaging, Macromolecules, 2014, 47, 1543-1552. [PDF]    

We report photoswitchable SP-PCL nanoparticles as a new fl uorescent probes in super-resolution imaging.

H. Q. Ma, H. Kawai, E. Toda, S. Q. Zeng, Z. L. Huang ^*, Localization-based super-resolution microscopy with an sCMOS camera Part III: Camera embedded data processing significantly reduces the challenges of massive data handling, Optics Letters, 2013, 38, 1769-1771. [PDF]        

A camera embedded data processing method for localization microscopy (LM) with faster detectors(such as sCMOS). This method  greatly reduce data volume(

C. Li ^§, W. L. Gong ^§, Z. Hu ^§, M. P. Aldred, G. F. Zhang, T. Chen, Z. L. Huang ^*, M. Q. Zhu ^*, Photoswitchable aggregation-induced emission of a dithienylethene–tetraphenylethene conjugate for optical memory and super-resolution imaging, RSC Advances, 2013, 3, 8967-8972. [PDF]    

We have demonstrated a novel and unique route to a highly erasable optical molecular switch which utilizes the photochromic switching of AIE-active DTE–TPE. The DTE–TPE conjugate is a novel fluorescent-switchable material.

F. Long, S. Q. Zeng, Z. L. Huang ^*, Localization-based super-resolution microscopy with an sCMOS camera Part II: Experimental methodology for comparing sCMOS with EMCCD cameras, Optics Express, 2012, 20, 17741-17759. [PDF]    

An experimental methodology for quantitatively comparing the performance of low-light cameras in single molecule detection and localization, and a newly launched sCMOS camera can present superior imaging performance than a EMCCD camera in the specific situation.

Y. N. Wang, T. W. Quan, S. Q. Zeng, Z. L Huang ^*, PALMER: a method capable of parallel localization of multiple emitters for high-density localization microscopy, Optics Express, 2012, 20, 16039-16049. [PDF]    

A fast high-density localization method for localization microcopy, which allows for fast super-resolution image reconstruction (>1000 molecules/ second) and a speed gain of up to 14 in temporal resolution of LM.
The imageJ plugin for this method is available now in the Software page.

H. Q. Ma, F. Long, S. Q. Zeng, Z. L. Huang ^*, Fast and precise algorithm based on maximum radial    symmetry for single molecule localization, Optics Letters, 2012, 37, 2481-2483. [PDF]    

A fast and high accuracy algeberic localization algoritm which does not rely on point-spread-function or noise model, and can executes ~1000 times faster than MLE with very similar precision.

Z. L. Huang ^§*, H. Y.  Zhu ^§, F. Long ^§, H. Q.  Ma, L. S. Qin, Y. F. Liu, J. P. Ding, Z. H. Zhang, Q. M. Luo, and S. Q. Zeng ^* , Localization-based super-resolution  microscopy with an sCMOS camera, Optics Express, 2011, 19, 19156-19168. [PDF]    

We evaluate the possibility of sCMOS camera in localization microscopy, and further characterize the noise performance of sCMOS.

T. W. Quan, H. Y. Zhu, X. M. Liu, Y. F. Liu, J. P. Ding, S. Q. Zeng, Z. L. Huang ^*, High-density localization of active  molecules using Structured Sparse Model and Bayesian Information Criterion, Optics Express, 2011, 19, 16963-16974. [PDF]    

A high-density localization algorithm, which is superior in processing single molecule images with weak signal embedded in strong background comparing with conventional sparse algorithm.

Z. G. Pei, L. S. Qin, Z. H. Zhang, S. Q. Zeng, Z. L. Huang ^*, Fiber-optic  system for monitoring fast photoactivation dynamics of optical highlighter  fluorescent proteins, Biomedical Optics  Express, 2011, 2, 2117-2125. [PDF]    

A simple and quantitative way to characterizing the photoactivation performance of optical highlighter fluorescent proteins, which relies on the measurement of small absorption spectra changes over time with a fiber-optic system.

J.  Liu, Z. G. Pei, L. Wang, Z. H. Zhang, S. Q. Zeng, Z. L. Huang ^*, A straightforward and quantitative approach  for characterizing the photoactivation performance of optical highlighter  fluorescent proteins, Applied Physics Letters, 2010, 97, 203701. [PDF]    

We show that light does dependent fluorescence curve, which is calculated from activation-intensity-dependent photoactivation and photobleaching rates, is capable of quantifying the photoactivation performance straightforwardly.

T.  W. Quan, S. Q. Zeng, Z. L. Huang ^*, Localization capability and limitation of electron-multiplying charge-coupled,  scientific complementary metal-oxide semiconductor, and charge-coupled devices  for superresolution imaging, Journal of Biomedical Optics, 2010, 15, 066005.     [PDF]    

Evaluating three typical low-light detectors(EMCCD, sCMOS, and CCD), we found that under some experimental accessible conditions, the sCMOS camera shows a competitive and even better performance than the EMCCD camera for superresolut.

T.  W. Quan ^§, P. C. Li ^§, F. Long, S. Q. Zeng, Q. M.  Luo, P. N. Hedde, G. U. Nienhaus, Z. L. Huang ^*, Ultra-fast, high-precision image analysis for  localization-based super resolution microscopy, Optics Express, 2010, 18, 11867-11876. [PDF]    

A fast image analysis method for real-time processing of experimental images without compromising localization precision or field of view.
   The imageJ plugin for this method is available now in the Software page.